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After a brief rinse in TBS, nuclei were counterstained with DAPI, followed by three washes in TBS (10 min each)

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In the present study, we examined changes in glucose metabolism in a mouse systemically harboring a loss of function mutation in Insr (a single amino acid substitution from proline to leucine at 1195 amino acid residue (P1195L)), which has been shown to act as a dominant-negative mutant in heterozygosity 6

In these experiments, researchers worked with human hair follicles outside the body (ex vivo) and with dermal papilla cells, which are special cells at the base of hair follicles that help control hair growth [4]

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