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After a 7-days acclimatization period, the mice were randomly assigned to five groups ( n = 8 per group), Normal Control (NC) group: did not undergo modeling induction

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4 The protective role of taurine in the pathological injury of the male reproductive system 4.1 The protective role of taurine in sperm preservation in vitro 4.1.1 Hypothermic preservation Semen preservation is the key to in vitro fertilization and artificial insemination, usually including hypothermic preservation (typically above 0C but below normothermic 32C to 37C mammalian temperatures) and cryopreservation(196 to 80 C) (53)

Study Population Participants were recruited from South Florida by Mailman Segal Center, by use of snowball sampling