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All lines were derived from skin fibroblasts that were reprogrammed using an integration-free episomal vector approach and characterized through karyotyping, expression of markers for pluripotent stem cells such as Nanog, Oct4, and SSEA4, and the capacity to generate cell types representative of all three germ layers of embryonic development (Kreitzer et al., 2013

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Biochem Biophys Res Commun 2004;314:92530

Many people dont recognize that needing to drink despite health risks, planning their medication timing around drinking schedules, or feeling anxious about periods without alcohol are warning signs of a substance use disorder

by Pam Helmlinger, RD, LDN, CDE