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J Biol Chem (2011) 286:12189201.10.1074/jbc.M110.174029 91 de BritoOMScorranoL

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A 30 L binding reactions were performed by the addition of labeled probe (0.5 g) and purified GST-IbRAP2.4 or GST protein (10 g) into the binding buffer (200 mM HEPES, 50 mM KCl, 5 mM DTT, 1 mM EDTA, and 20 pmol of poly dI-dC) and then allowed to proceed at room temperature for 30 min

Lithium regulates total and synaptic expression of the AMPA glutamate receptor GluR2 in vitro and in vivo