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Lyme disease is an infection rather than an autoimmune disease, but it can be accompanied by a substantial inflammatory immune response

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Ligand Coupling Most commonly, ligands are immobilized or coupled directly to solid support material by formation of covalent chemical bonds between particular functional groups on the ligand (e.g., primary amines, sulfhydryls, carboxylic acids and aldehydes) and reactive groups on the support

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After an antigen binds to a surface immunoglobulin, a series of events including proliferation and differentiation of that B cell results in secretion of Immunoglobulin that is the specific antibody for that antigen