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Non-commercially available metal-tagged antibodies were generated by conjugating each purified antibody to its respective metal isotope following standard procedures (Maxpar Antibody Labeling User Guide PRD002)

We diluted the siRNA transfection reagent with serum-free medium according to the reagent instructions to avoid the effect of serum on transfection efficiency

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Pharmacokinetic studies of several bioactive compounds have demonstrated limited oral bioavailability, rapid metabolism, extensive first-pass hepatic clearance, and low systemic exposure

The rationale is further supported by several studies that demonstrated that the human gut microbiome is a major determinant of plasma metabolome, potentially playing a more dominant role than genetics