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The discovery of its biological activity came from an unexpected observation: serum from young donors (age 20-25) could stimulate aged liver tissue to synthesize proteins at a rate characteristic of younger tissue, while serum from older donors (age 60-80) could not

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The culture broth was subjected to liquidliquid extraction with EtOAc in a 1:2 ratio at room temperature, with stirring at 100 rpm for 72 h

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2775S), chicken anti-TUJ1 antibody (1:500