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I.PereiraA

Binolfi, A

Equal protein loading was tested by stripping the membranes with 2% SDS, 100 mM -mercaptoethanol, 62.5mM Tris (pH 6.7) and then re-probing the membrane with antibodies to detect -actin (1:1000)

Vecchio et al., 2018)

Therefore, it is possible that prolonged exposure to high levels of inflammatory mediators, ROS and/or to drugs (particularly corticosteroids) in vivo may shape the metabolic activity of severe asthma eosinophils