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Tolerance was discussed somewhat separately from those topics, a finding that is intriguingly similar to a recent demonstration (in young-adult alcohol drinkers) that tolerance is a separate construct from all other criteria of an SUD (Watts et al., 2021)

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Experimental setup, binding, and elution conditions in affinity chromatography can vary greatly, so protocols should be optimized based on target protein

The measurements were performed according to the instructions provided with the ELISA kits for each parameter