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For sequential double labeling, the explants were incubated with the second primary antibody MYO7A overnight at 4 C, followed by incubation with the second secondary antibody for 1 h at room temperature

Med J Aust (1998) 168(8):4058

More water creates more dilute solution

(This latter model would predict that, unlike ACSL4 or POR deficiency, inactivation of such a ferroptosis executioner should not affect FIN-induced lipid peroxidation but would still drive ferroptosis resistance, thereby uncoupling lipid peroxidation from ferroptotic cell death

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