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24 h after seeding, cells were transfected with the plasmid containing the indicated construct using a standard polyethylenimine-based method

The yeast strain Saccharomyces cerevisiae has been engineered to incorporate five new unnatural amino acids (using the TAG nonsense codon and new, modified tRNA and tRNA synthetases) with keto groups that allow chemical modifications to the protein

A comparative analysis of metabolism and viability in porcine oocytes during in vitro maturation

Advantage, however, was obtained from heterogeneous populations, heterogeneous treatment protocols and incongruent biomarker reporting