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Lesson learned

Glycopyrrolate is available as a prefilled syringe for injection, commonly used in hospital and surgical settings for its anticholinergic properties

Briefly, 400 m slices were cultured on (Milipore, Billerica, Mass.) for 3 days after which they were biolistically transfected with tomato fluorescent protein (TFP) using a Helios Gene Gun (BioRad, Hercules, Calif.), according to the manufacturer's protocol, to visualize dendritic arbors