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WRAIR-5001 showed robust binding to a panel of clade 1a and 1b sarbecovirus RBD molecules with sub-nanomolar affinity and neutralized Delta, Omicron BA.1, Omicron BA.2, and SARS-CoV-1, but did not yield protection in the stringent K18 mouse model against a Delta VoC challenge, likely owing to the modest neutralization activity toward the Delta VoC

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Single-cell suspensions were labeled with fluorochrome-conjugated monoclonal antibodies (Table S22)