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Cyst(e)ine and GPX4 inhibition as therapeutic targets To exploit our findings in vivo, we first tested simultaneous inhibition of cysteine uptake and transsulfuration in vitro using IKE, an erastin analog with acceptable pharmacokinetic properties 40,41 , and PPG 33 (Fig

We therefore applied a combination of materials science characterization techniques to an in vitro model of bone-like tissue formation by human bone-marrow derived mesenchymal stromal cells (hMSC)

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Mobile phase for gel filtration was 200 mM NH 4 NO 3 at pH 7.5, prepared from TraceMetal Grade nitric acid (Fisher Scientific UK Limited, Leicestershire, UK) and ammonium hydroxide 25% solution (Honeywell Fluka, Seelze, Germany), which is compatible with ICP MS