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To investigate whether one or both putative binding sites were functional, we cloned fragments of 98 and 96 bp of the human IGF-1 3 UTR mRNA (NM_ 000618) containing the two putative miRNA-binding sites into the psiCHEK-2 vector (Supplementary Fig

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The study was approved by the national regulatory agency (the Federal Institute for Drugs and Medicinal Products [Bundesinstitut fr Arzneimittel und Medizinprodukte]) and the independent ethics committee of the Bavarian Chamber of Physicians