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Generation and validation of Mettl9 Deg mESC lines Deg For the generation of the Mettl9 Deg mESC lines, CAS9 was guided to the last exon of the endogenous Mettl9 locus, near the stop codon, by using an sgRNA designed with CRISPOR 161 and cloned into the px330-related px459 (Addgene) 149 gRNA/CAS9 vector

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