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If astrocytes are impaired, this breaks down

doi:10.1016/S0968-0004(00)01674-1

The medium was altered from the original formulation by substituting 0.2 M sodium sulfide (NaS) with 0.2 M ascorbic acid as the reducing agent and by supplementing it with additional carbon sources (5 mM each of acetate, propionate, and butyrate)

The backbone-cyclized ABLE protein ( CABLE ) was generated by connecting the C- and N-termini through engineered loop structures using the intein-mediated NCL of the expressed precursor (Fig