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Briefly, osteosarcoma cells with or without lentiviral infection (virus carrying GFP fluorescence) were harvested, washed twice with cold PBS, and resuspended in 1 binding buffer at a concentration of 1 10 6 cells/mL

Exosomal RNAs, such as miR-4443, modulate FSP1 expression, a glutathione-independent ferroptosis suppressor, altering cellular resistance to lipid peroxidation and contributing to disease outcomes [102] (Fig