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Peptides serve as delivery tags for CRISPR components, directing edits to targeted germ cell populations

Measurement of glutathione peroxidase and glutathione reductase activity To measure GPx activity, 120L of saliva were incubated in a final volume of 3mL with phosphate-EDTA buffer (100mM, pH 7.5, EDTA-Na2 1mM) in the presence of 30L of NADPH (20mM), 100L of GSH (60mM), and 4L (1IU) of GRd for 5min at room temperature

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