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Evidence favouring this hypothesis includes: (1) overproduction in INS-1E cells or in rat pancreatic islets of glutamate decarboxylase, an enzyme that decarboxylates glutamate in the cytoplasm, led to a reduced cellular glutamate content that correlated with the impaired GSIS [68]

Hepatocytes were plated at approximately 80% confluence and allowed to adhere for approximately 6 h before treatment