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The role of Zn in the development of ROP is controversial and not well studied but could affect the development of ROP by several mechanisms: (1) as a potent antioxidant

This in turn may lead to increased vascular tone (narrowing of vessels) and enhanced platelet aggregation, both of which may contribute to red cell stasis, sickling, and (in some instances) thrombosis

However, in the context of mitochondrial dysfunction, the balance between ROS production and antioxidant defense mechanisms is disrupted, leading to an excessive accumulation of ROS [10]

2010 Sep;64(9):933-9

At this point, in each well the buffer was replaced with 600 L uptake buffer containing compound and 0.12 Ci (80-110 mCi/mmol) of L-[3,3- 14 C]-cystine (Perkin Elmer) and incubated for 3 minutes at 37 C