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WT USP2 transfection, but not USP2 C276A, induced the protein level of TXNDC5 and decreased the TXNDC5 ubiquitination (Fig

Chromatin pellet was removed by centrifugation (1700 g at 4 C for 10 min), supernatant (soluble nuclear lysate) was saved and treated with 1.5 l of RNase A (Qiagen, 19101) at room temperature for 15 min

However, in the body, most of the actual norepinephrine reuptake results from the metabolite because its half-life is much longer than bupropions

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Levels may be lower in animals maintained primarily on hay compared with those on fresh forage