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However, maturation of erythrocytes and conversion of erythroblasts to mature erythrocytes during erythropoiesis is accompanied by enucleation and organelle clearance [32]

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extracellular signal-regulated kinase, ERK

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Cells were then washed twice in ice-cold mitochondrial isolation buffer (MIB: 20 mM HEPES, 70 mM sucrose, 220 mM mannitol, pH 7.4, 1 mM EDTA, Complete protease inhibitors), resuspended in MIB and lysed using nitrogen cavitation for 5 min at 500 psi