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Plates were washed five times with HBS + 0.1% Tween-20, and 100 l of streptavidinhorseradish peroxidase (HRP) was added to each well, followed by another one-hour incubation at room temperature

Apo- and mesohemerabbit HPX (90%) or proto-hemehuman HPX complexes (33.9 mM and 38.3 mM, respectively) were investigated

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Mitochondrial reactive oxygen species release is unaltered by IF In addition to determining changes in functional parameters, we measured the release of mitochondrial H 2 O 2 , a relatively stable and membrane-permeable ROS often used as a marker for oxidant production (Fig

Dose transitions should be medically supervised, with gradual titration to minimize nausea and allow metabolic adaptation