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10.1371/journal.pone.0081528 76 GuzmnM.BlzquezC

Note: Doses above 1.0 mL (100 units) require a 3 mL syringe with an appropriate subcutaneous needle (e.g., 2527G, inch) rather than a standard U100 insulin syringe

SIRT1, a deacetylase enzyme, plays a key role in regulating the central circadian rhythm in the SCN by promoting the transcription of the main circadian regulators, BMAL1 and CLOCK