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The sections were rinsed three times with double-distilled water and stained with Oil Red O solution (Sigma, O0625) for 15 min in the dark

Sources: Ionescu M, et al

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After 24 h, total protein extracts were obtained from whole flies homogenized in standard lysis buffer (20 mM Tris-Cl (pH 7.5), 200 mM NaCl, 2 mM EDTA, 10% glycerol, 1% Nonidet P-40 and EDTA-free Complete Protease Inhibitor Cocktail (Roche Diagnostics))