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One hundred microlitres of tenfold serial dilutions of EV-F3 in serum-free G-MEM was added to washed, confluent cells

Studies in mice suggest the potential usefulness of mesenchymal stem cell therapy in IPF treatment (Li, Han 2017)

This also supports the functional role of these enzymes in H 2 O 2 -scavenging, thereby implicating their importance in the plant defense

For chymotrypsin digestion the alkylated proteins were diluted to 1 M urea with 100 mM Tris and 10 mM CaCl2 buffer pH 8.0 (50 g of protease in 57 l added to 456 l of buffer) and treated with 2.5 g of chymotrypsin made fresh in 1 mM HCl

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