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The mechanism of action is due to the fact that Larazotide acts as an antagonist against zonulin, which is a protein in the small intestine that affects gut permeability

Chemosphere 332 , 138814 (2023)

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To increase the protein recovery, the pellet from this step was resuspended in a buffer (containing 7 M urea and 2 M thiourea in 0.4 M triethylammonium bicarbonate at pH 8.5), freeze-thawed, vortexed and centrifuged at 15,000 g for 15 min at 20 C