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After centrifugation (15 min, 12,000 g, 4 C), the upper aqueous phase was carefully collected and transferred into a new tube containing 1 ml of freshly prepared 70% ethanol solution, mixed by pipetting, and loaded onto a RNeasy spin column (RNeasy mini kit, Qiagen)

d3-carnitine were m/z 165.143.1 and 165.161.2

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These two peptides, when used together, have shown to increase growth hormone levels 5 times more than when administered alone