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Fixed cells were then washed three times with PBS and then permeabilized in 500 L 0.1% Triton X-100 in PBS for 10 min

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31 Predicting drug elimination based on half-life can go awry when very high doses of drugs are used, 32 as in the case of fluoxetine and the treatment of obsessive-compulsive disorder and when drugs are consumed in overdose

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Age-related changes in bone marrow mesenchymal stromal cells: a potential impact on osteoporosis and osteoarthritis development