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SOD-like catalytic activity test To catalytically eliminate O 2 radicals, potassium superoxide (KO 2 , 1 mg) was solubilized in dimethyl sulfoxide (DMSO) containing 18-crown-6-ether (3 mg/mL)

The cell suspension was passed through a 20 m filter and plated onto uncoated (bacteriological) petri dishes for 1 hour in incubator to remove residual microglia/astrocytes

One powerful and commonly used approach is to tag your protein of interest with Glutathione S-Transferase (GST) and perform a GST pull-down to see what binds to the protein of interest