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For western blot analysis, cells were collected after 72 h of transfection and lysed in RIPA buffer with protease inhibitor (Roche) and phosSTOP (Roche)

Subsequently, they observed a significant increase in serum alanine aminotransferase and lipopolysaccharide levels, along with the presence of liver steatosis

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The video was seen on television by tens of millions, which triggered the Thriller albums return to the phenomenal sales levels it had seen earlier in the year, and propelled the albums rebound to the top of the charts at the end of 1983, where it would remain through April 1984