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Hereditary spherocytosis HS is an inherited disorder characterized by the presence of spherical-shaped RBCs on peripheral blood smears and is most commonly associated with autosomal dominant inheritance [23]

Before performing the test, the normality of the data was inspected using a quantilequantile plot

The flow rate was 300 nL/min and linear gradient was 90 min (from 5 to 85% B over 67 min

The rapid accumulation of multiple isotopologues in metabolites of upper glycolysis and the pentose phosphate pathway (PPP) revealed that GAPDH inhibition by hydrogen peroxide induces rapid diversion of glycolytic flux into the PPP, and further, multiple rounds of cycling of carbons through the oxidative arm to amplify NADPH production and combat ROS (Fig

This unchecked oxidative stress further impairs energy production, damages blood vessels, and leaves the immune system exhausted and dysregulated, perpetuating the cycle of chronic illness