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One powerful and commonly used approach is to tag your protein of interest with Glutathione S-Transferase (GST) and perform a GST pull-down to see what binds to the protein of interest

Conformational changes due to ligand binding are known to be a major contributor to heat capacity changes 36 , and there is precedence for ligand binding to Bateman modules to diverge from a simple one-site binding model when dimerisation of Bateman modules occurs 37