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Donkey anti-goat and goat anti-biotin antibodies were diluted 1:10 in PBS, and the mixture was left to rotate overnight at 4 C

10.1016/s0022-2836(05)80154-8 8 BjrnestedtR.TardioliS.MannervikB

10.1124/jpet.108.141044 54

Han et al., 2016)

Alternatively, when using the standard curve method of analysis, an appropriate amount of Dynabeads magnetic beads can be added to each sample used to construct the standard curve.