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At higher serum levels, the effective half-life increases, promoting drug accumulation

Neurocritical Care (2nd ed.)

doi: 10.1111/j.1439-0396.2010.01104.x 274

The PCR reaction mixtures were subjected to the following conditions: pre-denaturation at 95 for 30 s, followed by 40 cycles of 95 for 5 s, 60 for 30 s