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When wild-type or mutant Cnd1 proteins under the control of the endogenous cnd1 promoter were expressed from LEU2-based plasmids, EMM-Leu medium containing 0.5 mM auxin was used to maintain the LEU2 plasmids

Arrigoni R, Arrigoni O

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Contrary to what some researchers and doctors have recommended, theres no evidence that raising blood levels of 25D above 50 ng/mL is beneficial, and theres some evidence that it may cause harm

This remarkable selectivity stems from 5-amino-1MQs specific interaction with the nicotinamide-binding pocket of NNMT, rather than the SAM-binding site common to many methyltransferases