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Founder mice with the modified Dmrta2 2xHA allele were screened for appropriate integration of the transgenic sequences at the knock-in region as well as for the presence of predicted ( potential off-targets followed by PCR followed by Sanger sequencing of the PCR products (Genewiz)

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221,222 IKK phosphorylates IB, which subsequently undergoes ubiquitylation and degradation, inducing NF-B and inflammatory reactions

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