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Most Raw264.7 cells were polarized into the M1-like phenotype upon LPS stimulation, evidenced by the high expression of CD86 (phenotype marker of M1-like macrophages)

Prior to staining, cryopreserved PBMCs were thawed in a water bath at 37 C and washed twice in thawing media (RPMI 1640 and 10% inactivated FBS) containing 1:10,000 Pierce Universal Nuclease (Thermo Scientific, Waltham, MA, USA)

Kurz MA, Boyer TD, Whalen R, Peterson TE, Harrison DG

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Crude Blueberry phenolic extracts improve gut barrier integrity and exert anti-inflammatory and antimicrobial activity in an in vitro weaning stress model