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To further increase the ERG yield, we focused next on reinforcing the sulfur metabolic flux toward ERG biosynthesis, because this pathway was indicated to be the rate-limiting pathway in our previous study 26

Acetaldehyde and ROS generated during alcohol metabolism can directly stimulate KCs and HSCs, promoting the transcription and secretion of TNF- [124, 125]

Future work should include expert-driven evaluations of the identified KERs using the BH criteria, covering aspects such as biological plausibility, essentiality, empirical support (e.g., doseresponse and temporal patterns), and overall coherence across studies

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