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Retrieved September 22, 2021, from

PMID: 23846913 Braganza J.M

ROS production and glutathione response in keratinocytes after application of -carotene and VIS/NIR irradiation

Iskander, J., Strikas, R

For phospho-flow cytometry analysis, to preserve the fluorescent proteins, cells were fixed with 2% paraformaldehyde for 30 min at room temperature, followed by permeabilization with 90% ice-cold methanol for 30 min and then staining in 1 permeabilization buffer (eBioscience) for 30 min at room temperature