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Following trypsinization, cells were counted and plated as single cells in a 6-well dish ( 5001000 cells per well)

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They found that carnitine administration was associated with a significant reduction in BMI, and waist and hip circumferences compared to placebo

Plasmid expressing the SH3 domain of Src (residues 83-141) was designed by inserting a C-terminal FLAG-tag and a stop codon in pET28a-3D-Src

Handbook of Behavioral Neuroscience