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Another 1 ml aliquot of the supernatant was neutralized with 1.5 ml of 0.5 M phosphate buffers, 50 l of 2-vinylpyridine was added to mask GSH, and the constants of the tube were mixed until an emulsion formed

Melatonin attenuates inflammatory bone loss by alleviating mitophagy and lactate production

[Products in use] Extracellular LDH : Cytotoxicity LDH Assay Kit-WST (Product code: CK12) Phosphatidylserine: Annexin V Apoptosis Plate Assay KitProduct code: AD12 Cell viability : Cell Counting Kit-8 (Product code: CK04) Intracellular Fe 2+ : FerroOrange (Product cose: F374) *Normalized with Hoechst 33342 fluorescence intensity Lipid peroxidation : Lipid Peroxidation Probe -BDP 581/591 C11- (Product code: L267) [Experimental conditions] Cell type: HepG2 cell(210 4 cells/well) Drugs: Staurosporin(5 mol/l), Erastin(25 mol/l), Erastin+RSL3(both 25 mol/l) *Diluted in serum-free medium

Abstract Glucose 6-phosphate dehydrogenase deficiency (G6PD-d) is the most common enzymopathy in the world, occurring in 58% of the global population (half a billion people)

Inflamm Res 62(4):425440