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Phase III reaction: Product transport across the canalicular membranes Transporters facilitate drug-product excretion into the bile Bile An emulsifying agent produced in the liver and secreted into the duodenum

CoQ transports electrons to complex III

After staining, slides were rinsed with DI water, dehydrated, and mounted using toluene (Fisher Scientific, USA)

Surface area examination of particles in the liquid phase may be accomplished with simple titration, but this requires a lot of manual work

Cytochemical SA--galactosidase assay Cells were seeded in six-well plates, fixed with 0.5% glutaraldehyde (Sigma, G5882) in PBS for 15 min, washed with 1 mM MgCl 2 /PBS (pH 6.0) and then incubated with X-Gal staining solution (1 mg ml 1 of X-Gal (Thermo Scientific, 2737087), 5 mM K 3 (Fe(CN) 6 ) and 5 mM K 4 (Fe(CN) 6 ) for 68 h at 37 C