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Chondrocytes and NPCs demonstrated inward cell growth, viability, and tissue production on both acrylonitrile butadiene styrene and PLA scaffolds, which were printed using a simple and cost-effective desktop 3D printer

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The cells were allowed to self-organize into aggregates for up to 24 hours on an orbital shaker at 70 RPM in a humidified 37 C, 5% CO 2 incubator

A 488 nm laser line was used to excite green fluorescence, and emissions for GFP were collected in the 500550 nm range