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After several washes with Tris buffer, the purified envelopes were re-suspended in the buffer containing 2% Triton X-100 and 0.02% sodium azide

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The cysTMT approach utilizes two different isobaric tag groups with two different purposes: the cysTMT tags are used to label thiol groups on Cys in up to six samples (126, 127, 128, 129, 130, and 131 m/z ), while the iTRAQ tags are used to label N-terminal and lysine residues of peptides in up to six various samples (114, 115, 116, 117, 119, and 121 m/z )

ALD and ALD+ had more hepatorenal syndrome, esophageal varices with bleeding, and spontaneous bacterial peritonitis than MASLD and METALD* (Table 1)

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