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1:1000 dilution), and Anti-GAPDH (Santa Cruz Biotechnology

doi: 10.1093/emboj/cdf524 78 SchiefelbeinJ

Rep 77 , 14911513 (2025)

Induction of the MPTP compromises mitochondrial ATP production, depolarizes the mitochondrial membrane, and consequently shuts down mitochondrial function6668

The number of surviving hair cells was counted using a semi-automated deep learning method integrated into Fiji according to the previously described procedure [37]