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But it's very much tightly regulated in like, this is how the protocol works, this is how we do things

Light micrographs of the cross-sectioned sciatic nerve from the Cont (A, B)

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We noted decreased levels of GATA1 mRNA levels on days 11 and 13 following USP7 knockdown ( Online Supplementary Figure S3A, B ), likely due to the fact that GATA1 mediates its own regulation at the late stages of erythropoiesis.3736 Similarly, inhibition of USP7 activity by the USP7-specific inhibitors P5091 and P22077 also resulted in significant decreases in GATA1 protein levels in a dose-dependent manner (Figure 2C, D), with no obvious effects on other transcription factors beside a slightly decreased expression of KLF1 ( Online Supplementary Figure S4 ), implying that USP7-mediated regulation of GATA1 depends on the enzymatic activity of USP7