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Dug1p assay Dug1p was pre-incubated with varying concentrations of juglone for 30 min and later the substrate, cys-gly dipeptide (2.5 mM) was added to the reaction mixture and incubated for an hour

Of note is the degradation of CDK2 in HL-60 cells with DC 50 values of 12.6 nM and D max of 82%