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To inhibit autophagy, vitiligo melanocytes were grown in M254 containing HMGS and treated with the autophagy inhibitor 3-methyl adenine (5 mM) (Sigma Aldrich) for 24-48-72-96 h whereas vitiligo fibroblasts were cultured in DMEM with 10% FBS and incubated with the inhibitor 3-methyl adenine (5 mM) (Sigma Aldrich) for 24 and 48 h

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Therapeutically, targeting adaptive immune pathwayssuch as restoring regulatory T cell (Treg) function, modulating Th17/Th1 responses, or influencing antigen presentationrepresents a potential adjunctive strategy to complement microbiome-directed and neuroprotective approaches, although its clinical efficacy remains to be fully established [88]

Clc exhibited around 20% higher activity than Np and also outperformed both ClcN 145 Q and ClcXGR3 (Fig

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